The biosynthesis of natural products by heterologous\nexpression of biosynthetic pathways in amenable production\nstrains enables biotechnological access to a variety\nof valuable compounds by conversion of renewable resources.\nPseudomonas putida has emerged as a microbial laboratory\nwork horse, with elaborated techniques for cultivation and\ngenetic manipulation available. Beyond that, this bacterium\noffers several particular advantages with regard to natural\nproduct biosynthesis, notably a versatile intrinsic metabolism\nwith diverse enzymatic capacities as well as an outstanding\ntolerance to xenobiotics. Therefore, it has been applied for\nrecombinant biosynthesis of several valuable natural products.\nThis review provides an overview of applications of P. putida\nas a host organism for the recombinant biosynthesis of such\nnatural products, including rhamnolipids, terpenoids,\npolyketides and non-ribosomal peptides, and other amino\nacid-derived compounds. The focus is on de novo natural\nproduct synthesis from intrinsic building blocks by means of\nheterologous gene expression and strain engineering. Finally,\nthe future potential of the bacterium as a chassis organism for\nsynthetic microbiology is pointed out.
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